The biosynthesis of 2H-labeled phenylalanine was done by converse of low molecular weight substrates in a new RuMP facultative methylotrophic mutant Brevibacterium methylicum. Isotope components of growth media and characteristics of bacterial growth.
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The production of 2H-labeled amino acids by a new mutant of RuMP fucultative methylotroph Вrevibacterium methylicum Oleg V. Mosin1 1 Department of Biotechnology, M. V. Lomonosov State Academy of Fine Chemical Technology, Vernadskogo Prospekt 86, 117571, Moscow, Russia Summary The biosynthesis of 2H-labeled phenylalanine was done by converse of low molecular weight substrates ([U- 2H]methanol and 2H2O) in a new RuMP facultative methylotrophic mutant Brevibacterium methylicum. To make the process work, adapted cells with improved growth characteristics were used on minimal medium M9 with the maximum content of 2H-labeled substrates. Alanine, valine, and leucine/isoleucine were produced and accumulated exogeneously in addition to the main product of biosynthesis. Electron impact mass spectrometry of methyl esters of the N-Dns-amino acid mixture obtained after the chemical derivatization of growth medium with dansyl chloride and diazomethane, was done to calculate the deuterium enrichment of the amino acids synth
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