The course of multiple sclerosis in patients, which is associated with the characteristics of reception of sex steroids in the central nervous system in men and women. Odds in allergic encephalomyelitis in mice of different sex and castrated animals.
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GENDER FEATURES OF CLINICAL COURSE OF EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS IN LABORATORY ANIMALS Predtechenskaya E.V. Sorokina I.V. Zaigraev V.Y. Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of central nervous system (CNS). MS incidence is 3,6 per 100 000 in females and 2 per in males. [1, 2] Pathogenesis of brain tissue injury in MS lesions is not completely known. For now, 4 pathohistological patterns are described. They reflect different types of MS course and their pathogenesis may be different. [3] There is substantial difference in the course of MS between patients with different sex. Thus, the female-to-male ratio in MS incidence is 2,3 and increases for 6% per every 5 years [2] Аge of the MS debut is younger in females, also females usually have relapsing-remitting course of the disease unlike males who have primary-progressive course. [1, 4] There is widely described protective role of pregnancy in MS. The explanation for this phenomenon is the neuroprotective role of high-leveled pregnancy steroid hormones, particularly estriol. [10] So, these gender features suggests that steroids have great influence for course of the MS. There is some studies on the treatment of MS using sex steroids. [5, 6] Experimental autoimmune (allergic) encephalomyelitis (EAE) is a most commonly used animal model for MS. It can be induced on various animal species, particularly in the mouse by immunization using myelin basic protein (MBP), proteolipid protein (PLP) or myelin oligodendrocyte glycoprotein (MOG). EAE pathogenesis is based on T-cell mediated immune inflammation that substantially comply with pathogenesis of MS. [3, 7] Different mouse strains in combination with different antigens can cause or remitting-relapsic or monophasic or chronic course of EAE. [8] Course of MOG35-55-induced EAE in C57BL/6 mice should be monophasic and self-limited. [9] So, EAE is a suitable model for studying of the demyelination and spontaneous remyelination and factors that can influence on it. The purpose of our study is evaluation of clinical features of clinical course of EAE in female, male and castrated male C57BL/6 mice. Features of clinical course was evaluated by three parameters: · Day of the disease debut · Clinical phase duration · Total severity degree Methods Animals Male, female, and castrated male C57BL/6 mice (n=48, 5-7 weeks of age, 20-28 g of weight) were raised in Novosibirsk institute of Organic Chemistry, Siberian Branch of the Russian Academy of Science (Novosibirsk, Russia). All experimental procedures were approved by the Institutional Animal Care Committee. 12 days before experiment starts, 16 male mice were castrated. Animals were separated for 3 groups: males, castrati, females (n=16 in each; 10 experimental mice and 6 intact for control).
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