Estimate of frequency prevalence of alleles of the DRD4 gene in nomadic and urban residents of the north of Western Siberia. Preparation of samples of buccal epithelium cells for polymerase chain reaction. The essence of finding differences in children.
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Сибирский государственный медицинский университет DRD4 GENE POLYMORPHISM IN CHILDREN OF NOMADIC AND URBAN INDIGENOUS POPULATIONS IN THE NORTH OF WEST SIBERIA Ильинских Н.Н. Dopaminergic system DRD4 gene are associated with cognitive human activity [10]. Repeat region are found in exon 3 of this gene. The number of repetitions can be from 2 to 11. Particular attention is drawn to a DRD4*7R allele with 7 repeats emerged in humans according to some scientists [7, 12] about 40,000 years ago during the migration of modern man from Africa to Eurasia. A study of Brazilian scientists [11] have demonstrated that this allele is very often present in the genotype of Native American nomadic tribes of “hunter-gatherers” and much less often it is observed in the sedentary Native Americans. Perhaps just such features as “novelty seeking” and “perseverance” which are typical for the “gatherers” with DRD4*7R [8] give them advantage in their nomadic lifestyle. The advantage is likely to disappear when a person moves to a settled way of life and undergoes “selection”. However, it was found that the DRD4*7R allele is much less common in the Mongoloid populations than it is observed in European and American populations [6]. Our previous study have shown significant differences the frequency of DRD4*7R allele between the students who came to study from the northern regions of West Siberia [2]. The aim of the work was to assess prevalence of the frequencies of the DRD4 alleles in nomadic and urban residents of Yamal-Nenets Autonomous Area (YaNAA) of Tyumen Region belonging to different ethnic groups. Material and methods We examined schoolchildren belonging to different indigenous ethnic groups including Nenets, Selkups, and Khanty whose parents either resided in the local settlements or lead a nomadic way of life rearing their reindeer herds in YaNAA of Tyumen Region. The study was performed in the boarding schools of Purovsky district of YaNAA where were both the children of nomadic people (ChNP) and the children whose parents permanently lived (ChPLP) in a settlement. The samples of buccal epithelial cells for the polymerase chain reaction (PCR) were obtained from 178 of the ChNP and 140 of the ChPLP. Informed consents were received from the children’s parents or their guardians to carry out this research. The schoolchildren were also divided into subgroups based on ethnicity (Nenets, Selkups, and Khanty). The samples of DNA were extracted from the buccal epithelial cells by means of reagents and a technique developed by PrepFiler Automated Forensic DNA Extraction Kit (USA). Statistically significant difference between the samples was considered in p
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