A feature of the use of filar and stage micrometers for determining the rate and scale in biological cells and tissues. The characteristic of measuring the size of cells in a microscopic slide using the universal instrument of the ophthalmic eyepiece.
LUBLIN UNIVERSITY OF TECHNOLOGY ENVIRONMENTAL BIOLOGY FUNDAMENTALS OF MICROSCOPY Lublin 2015 Aim of Classes Focusing on microscope technique and using filar and stage micrometers to determine size and scale in biological cells and tissues. Apparatus and Materials Microscope , filar eyepiece micrometer,stage micrometer , sharp knife or scalpel, small onion , slides and cover slips , forceps, mounted needle , tweezers, dropping pipette, 0,85%NaCl solution 1. Preparing a slide of onion epidermal cells 1. Cleaning a slide 2. Placing a drop of 0,85% NaCl solution in the center of the slide. 3. Cutting open an onion and separating some of the leaves, peeling off one of the thin layers of epidermal tissues from the inner concave surface of a leaf. 4. Transferring sample to a drop of 0,85 % NaCl solution on a microscope slide - using tweezers and a mounted needle to making sure that the tissue is not folded. filar micrometer tissue ophthalmic 5. At an angle , placing one slide of the cover slip against the slide maki
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